荧光定量PCR法检测点带石斑鱼神经坏死病毒研究     

彭智发 《福建畜牧兽医》2015,(1)

根据己公布的神经坏死病毒全基因序列,应用引物设计软件依据Gene Bank所收录的各种石斑鱼神经坏死病毒RNA2基因组全序列选择保守区域,根据Taqman探针引物设计原则,设计Taq Man探针及其引物。采用Taq Man探针技术,并且将假病毒技术构建的含有神经坏死病毒RNA2基因组的MS2噬菌体假病毒作为阳性质控品,建立Taq Man探针检测点带石斑鱼神经坏死病毒的荧光定量PCR方法,拟合标准曲线,能够在病毒检测中进行绝对定量,能检测到10个病毒拷贝模板数,在临床检测中得到很好运用。本研究建立实时荧光定量RT-PCR,有助于日常检验工作,为鱼类神经坏死病毒的筛选和监测提供基础,有助于控制神经坏死病的疫情传播。  相似文献   

外源人绒毛膜促性腺激素诱导黑边石斑鱼排卵的研究     

徐文刚  唐永政  张建柏  征矢野清  邹华锋 《广东农业科学》2020,47(4):122-128

【目的】了解外源人绒毛膜促性腺激素(human chorionic gonadotropin,HCG)诱导黑边石斑鱼(Epinephelus fasciatus)排卵的影响。【方法】以成熟的黑边石斑鱼为研究对象,测量其体长、体质量和肥满度后,在2018年7—8月,使用HCG激素(剂量为200 IU/kg或500 IU/kg)注射分别对6尾雌鱼进行成熟诱导。【结果】在200 IU/kg HCG处理中,注射24 h前有5尾雌鱼的卵母细胞处于第三次卵黄球前期阶段;注射48 h后2尾雌鱼出现核移动卵母细胞,1尾雌鱼的卵母细胞为第三次卵黄球后期阶段;注射60 h后1尾雌鱼出现排卵现象,其余5尾雌鱼均为第三次卵黄球后期阶段。在200 IU/kg HCG处理的排卵个体中,注射时其卵母细胞直径为477.0 μm,注射后60 h卵母细胞的直径增加至624.4 μm。此外,经催产、排卵、受精和孵化后共获得总卵数16 906粒,受精率为68.7%,孵化率为43.0%。而500 IU/kg HCG处理的黑边石斑鱼排卵失败。【结论】使用剂量为200 IU/kg的外源HCG激素可诱导黑边石斑鱼成熟且排卵,但为了提高排卵率、受精率和孵化率,其注射剂量和效应时间还需要进一步调节。  相似文献   

5种石斑鱼遗传差异的RAPD分析   总被引：3，自引：0，他引：3  

区又君  吴勇  李加儿  刘楚吾 《南方水产》2008,4(2):56-62

运用随机扩增多态性DNA（random amplified polymorphic DNA,RAPD）技术对鞍带石斑鱼（Epinephelus lanceolatus）、驼背鲈（Cromileptes altivelis）、棕点石斑鱼（E.fuscoguttatus）、斜带石斑鱼（E.coioides）和鲑点石斑鱼（E.fario）的遗传多样性及系统发生进行了研究。从120个RAPD随机引物中筛选出扩增效果好的引物19个,分别用于5种石斑鱼基因组DNA的扩增。结果显示,5种石斑鱼平均多态性位点比率（P）分别为64.23%,72.61%,60.34%,69.97%,73.94%;个体间平均遗传相似系数（S）分别为0.8238,0.8110,0.8345,0.8277,0.8064;平均遗传距离（D）分别为0.1762,0.1890,0.1655,0.1723,0.1936;平均Nei基因多样性指数（H）分别为0.1189,0.1364,0.1028,0.1439,0.1648;平均Shannon信息指数（Hi）分别为0.1801,0.1992,0.1530,0.2100,0.2434。5个种间的遗传距离（Dxy）在0.3964～0.6085之间。  相似文献   

中国南部养殖石斑鱼“肿胀病”的研究     

刘荭  江育林 《华中农业大学学报》2002,21(3):273-277

1998年5月至7月间,在中国南部养殖石斑鱼暴发"肿胀病",引起石斑鱼大量发病、死亡.从体表和鳍条的溃疡病灶处分离出2个菌株.形态学观察和生化试验结果表明这2个菌株均为鳗弧菌.将肝、脾、心和肾的上清液接种到CO、FHM、CAB、CK、PG和PC细胞上,没有观察到细胞病变.患病鱼的腹腔中有大量黑色的球状物和腹水,使患病鱼呈现明显的腹部肿胀的症状.组织病理学观察结果表明,这些黑色的球状物由多核细胞组成的增生性组织.  相似文献   

大川河虎嘉鱼产卵场条件及其产卵习性的初步研究     

周仰璟  吴万荣 《水产学杂志》1988,(1)

本文报导了大川河虎嘉鱼的产卵时期、产卵群体、性比组成、产卵行为及其产卵场分布、产卵场的理化因子和环境条件,并阐述了产卵场分布、产卵习性与生态条件的关系,通过研究表明大川河虎嘉鱼产卵场分布在具有急洄流水温4.5—9℃、底质砂砾石、水质清澈、着生硅藻和水生昆虫幼虫丰富的大川河上游,全长约9.5公里,面积约为330亩,产卵期3月中下旬,产卵群体性比1:1。  相似文献   

布氏哲罗鲑年龄与生长的初步研究   总被引：9，自引：1，他引：9  

吴万荣 《水产学报》1987,11(1):37-44

本文根据鳞片生长环纹的计数,并同鳞片、脊椎骨、鳃盖骨等上的年轮相对照,对4龄内的布氏哲罗娃的年龄与生长进行了研究。其结果摘要如下:(1)布氏哲罗鲑鳞片上的环纹随年龄而变化:每月长出二圈生长环线。鳞片上具有明显的“切割”年轮特征;脊椎骨、鳃盖骨上具有明带与暗带(线)形成的年龄特征。(2)布氏哲罗鲑在4龄内,体长在年龄上的回归方程为L=189.9468t+26.7934(r=0.991,df=35);体重年龄的回归方程为W=6.2451.e~(21219t)(r=0.978,df=35);鳞片半径与年龄的回归方程为R=0.7425t—0.1596(r=0.980,df=35);体重和体长的回归方程为W=6.7717357×10~(-6)L~(3.0740)(r=0.999,df=35);体长与鳞片半径的回归方程为L=251.0540R+71.2288(r=0.991,df=35)。(3)布氏哲罗鲑在4龄内,体长平均年增长为189.95mm;并随年龄增大而下降;生长常数、生长指标、肥满度系数等随年龄增大而增大。  相似文献   

Alternative vegetable lipid sources in diets for grouper, Epinephelus coioides (Hamilton): effects on growth, and muscle and liver fatty acid composition     

Hei-Zhao Lin  Yong-Jian Liu  Jian-Guo He  Wen-Hui Zheng  & Li-Xia Tian 《Aquaculture Research》2007,38(15):1605-1611

The aim of this study was to investigate the effects of different oils on growth performance and lipid metabolism of the grouper, Epinephelus coioides. Five experimental fish meal‐based isonitrogenous and isolipidic diets were formulated containing either 5.5%‐added fish oil (FO), soybean oil (SBO), corn oil (CO), sunflower oil (SFO) or peanut oil (PO). Each diet was fed to triplicate groups of 20 fish (initial body weight 13.2±0.02 g) grown in seawater at 28.0–30.5 °C for 8 weeks. Fish were fed twice a day to visual satiety. No significant differences in the survival, weight gain, specific growth rate, feed conversion ratio, protein efficiency ratio or hepatosomatic index were found between fish fed the FO or vegetable oils (VO) diets. Dietary lipid sources did not affect whole‐body composition among grouper fed the various diets. Muscle of fish fed the FO diet had significantly higher levels of 14:0, 16:0, 16:1n‐7, 20:5n‐3[eicosapentaenoic acid (EPA)] and docosahexaenoic acid (DHA)+EPA (except for PO fed fish) compared with those of fish fed VO diets. However, the levels of 18:1n‐9, 18:2n‐6 and DHA/EPA ratios in the muscle of fish fed FO diet were significantly lower than those of fish fed the VO diets. The liver of fish fed the FO diet had significantly higher levels of 18:0, 20:5n‐3, 22:6n‐3, n‐3 highly unsaturated fatty acids and DHA+EPA than those of fish fed the VO diets, whereas increases in 18:1n‐9, 18:2n‐6 and mono‐unsaturated fatty acid levels were observed in the liver of fish fed the VO diets.  相似文献   

Optimal dietary protein requirement of grouper Epinephelus coioides juveniles fed isoenergetic diets in floating net cages   总被引：3，自引：0，他引：3  

Z. Luo  Y.J. Liu  K.S. Mai  L.X. Tian  D.H. Liu  & X.Y. Tan 《Aquaculture Nutrition》2004,10(4):247-252

An experiment to determine the optimal protein requirement of grouper Epinephelus coioides juveniles was conducted in floating net cages (1.5 m × 1 m × 1.5 m). Six isoenergetic fishmeal–casein‐based experimental diets containing 350–600 g kg^?1 crude protein (CP) were fed to triplicate groups of 20 fish (10.7 ± 0.2 g) for 56 days. Weight gain (WG) and specific growth rate (SGR) increased with increasing dietary protein level from 350 to 450 g kg^?1 and then plateaued above these levels. Feed intake (FI) showed no significant difference among fish fed more than 350 g kg^?1 CP. Lowest feed conversion ratio (FCR) was found for fish fed 500 g kg^?1 CP but this was not significantly different from that of fish fed the 450 and 600 g kg^?1 CP. Lowest protein efficiency ratio (PER) was found for fish fed 550 and 600 g kg^?1 CP. Fish fed the 600 g kg^?1 CP had the highest body protein and moisture contents but the lowest body lipid content. Body ash content was unaffected by protein level for fish fed >400 g kg^?1 CP. Dietary protein level had no significant effect on hepatosomatic index (HSI). Fish fed the 350 g kg^?1 CP had significantly lower condition factor (CF) and viscerosomatic index (VSI). Based on broken‐line regression analysis of SGR the optimal dietary protein requirement for E. coioides juveniles was determined to be close to 480 g kg^?1.  相似文献   

福建南部养殖石斑鱼匹里虫(Pleistophora sp.)病观察     

陈信忠  龚艳清  苏亚玲  俞秀霞  黄丽莎 《福建水产》2005,(1):30-33

近年来，福建南部海区一些网箱养殖石斑鱼常见一种流行性孢子虫病，病鱼主要表现为游动失常、厌食、消瘦。外表无明显病理症状，部分病鱼腹部膨大变形，严重者可引起死亡。剖检可见腹腔、生殖腺、肝脏、结缔组织以及肌肉组织内散布或成串排列数个到数十个大小不一的黑色或银灰色孢囊。包囊内含大量孢子虫的子孢子，子孢子呈圆形或椭圆形，大小约4～5μm。根据形态学以及寄生特点确定该寄生虫是一种匹里虫。  相似文献   

Nucleotide sequence and mRNA expression analysis of <Emphasis Type="Italic">β</Emphasis>-actin gene in the orange-spotted grouper <Emphasis Type="Italic">Epinephelus coioides</Emphasis>     

W.?M.?ZhangEmail author  Y.?Zhang  L.?H.?Zhang  S.?G.?Wang  T.?Y.?Zhu  D.?Lin  G.?Z.?Ma 《Fish physiology and biochemistry》2005,31(4):373-383

The full-length cDNA, encoding the orange-spotted grouper β-actin and spanning 1920 bp including a poly (A) tail, was cloned from its brain cDNA library. The open reading frame encodes a protein of 375 amino acids. Sequence analysis indicated that it contained the typical structural features of cytoplasmic actins, and showed higher homology with other vertebrate β-actin than any other members of the actin family. The partial genomic sequence indicated that the organization of the β-actin gene in the orange-spotted grouper might also be conserved. Northern blot analysis indicated that it was expressed at high levels in the brain, spleen, adipose tissue, ovary, and liver, but at low levels in the gill filament and heart, and at a very low level in the kidney. The expression of β-actin gene in the skeletal muscle was barely detectable. These results indicated that the expression of the orange-spotted grouper β-actin gene showed significant variation in different tissues. Therefore, caution should be taken when using β-actin gene as an internal control in the normalization of gene expression among tissues. Whereas, semi-quantitative RT-PCR analysis indicated that treatment with 17α–methyltestosterone (MT) had little effect on the mRNA expression of β-actin gene in the in vitro incubated hypothalamus, pituitary, and ovary fragments of the orange-spotted grouper, suggesting β-actin can be used as an internal control for RT-PCR analysis of MT effects on gene expression in these tissues.  相似文献